Extended Data Fig. 2: Inhibitor selectivity for PLK4—and not other kinases—is required for the synthetic lethal killing of cells overexpressing TRIM37.
From: Targeting TRIM37-driven centrosome dysfunction in 17q23-amplified breast cancer
a, Representative data of a 10-day clonogenic survival of indicated MCF-7 cell lines treated with DMSO (control), centrinone, CFI-400945 or ZM447439. Data acquired in parallel to experiment in Fig. 1c, d. b, Quantification of a, n = 3 biological replicates. P values, unpaired two-tailed t-test. Mean ± s.e.m. c, Left, representative flow cytometric analysis of DNA content in MCF-7 cells treated with DMSO (control), centrinone, CFI-400945 or ZM447439 for 3 d. Right, quantification of the percentage of cells with >4N DNA content (polyploidy). n = 3 biological replicates. Mean ± s.e.m.
