Extended Data Fig. 5: RNA sequencing of sRNAs from PA14.
From: C. elegans interprets bacterial non-coding RNAs to learn pathogenic avoidance
a, sRNA training protocol using RNA isolated from PA14 cultures grown at 25 °C or 15 °C on plates, or in liquid culture. b, Principal component analysis of PA14 sRNA sequencing. c, Upregulated sRNAs in 25-°C grown PA14 compared to 15 °C-grown and liquid-grown PA14 sRNAs. The outermost grey circle represents the PA14 genome, with previously identified sRNAs that were upregulated (DESeq2, adjusted P ≤ 0.05) in the 25-°C plate condition relative to 15-°C plate grown PA14 (pink) or liquid-grown PA14 (green) are indicated. Overlapping sRNAs upregulated in the 25-°C plate condition relative to both other conditions are noted (blue dots). sRNAs annotated with white lines. d, Venn diagram showing the number of PA14 sRNAs upregulated at 25-°C growth conditions compared to liquid growth (blue circle) or 15-°C growth (red circle). The six shared upregulated sRNAs are shown.
