Extended Data Fig. 4: PARP2 interaction with nucleosomes.

a, The PARP2–HPF1 subset comprising 140,000 particles (left) was further classified and refined. The final map containing two PARP2–HPF1 complexes (middle) was refined to 6.3 Å. FSC curve is shown on the right. The map is coloured by local resolution. b, Model of activated PARP2–HPF1 (Fig. 1a) was rigid body fitted into cryo-EM map of two PARP2–HPF1 complexes bridging two mononucleosomes. c, Schematic representation of PARP2 and HPF1 organization is shown on the left. On the right, PARP2–HPF1 model is coloured by domains. d, Map quality and fit of the model are shown for the region shown in Fig. 1d. e, Alignment of PARP2–HPF1 bridging two nucleosomes with 5′-phosphate (5′ P) DNA (PARP2 WGR domains are shown in violet and pink, DNA in grey) with the X-ray structure of isolated PARP2 WGR domains bound to double-strand DNA with 5′ P (yellow; PDB 6F5B). f, Alignment of PARP2–HPF1 bridging two nucleosomes with 5′ P DNA, with the X-ray structure of isolated PARP2 WGR domains bound to double-strand DNA with 5′ P. The model is coloured by r.m.s. deviation (r.m.s.d.). g, Map quality and fit of the model are shown for the regions shown in Fig. 1e.