Extended Data Fig. 4: Plasma membrane profiling by tandem mass tag (TMT)-based MS3 mass spectrometry.
From: Red blood cell tension protects against severe malaria in the Dantu blood group
The impact of the Dantu polymorphism on the levels of proteins expressed on RBC membranes was quantified using mass spectrometry. a, Hierarchical cluster analysis of all membrane proteins, quantified and annotated as described in the Methods. Fold change was calculated for each donor by: (signal-to-noise (donor)/average signal-to-noise (non-Dantu)). b, Proteomic quantification of the markers shown in Fig. 2a (three biologically independent samples per genotype group). All markers except for GYPB were quantified by proteomics. Statistical comparisons of quantitative protein expression across Dantu genotype groups were performed using a two-tailed t-test with Benjamini–Hochberg multiple hypothesis correction: *P < 0.05, **P < 0.01. All P values are listed in Supplementary Table 4.
