Extended Data Fig. 11: Trim-Away experiments in cow and human embryos.
From: Initiation of a conserved trophectoderm program in human, cow and mouse embryos
a, b, Schematic of the Trim-Away experiments. c, Immunofluorescence analysis of anti-mouse IgG secondary antibody to detect the electroporated mouse anti-aPKC primary antibody, rabbit anti-aPKC primary antibody to detect the aPKC protein, and DAPI nuclear staining in human morula embryos in control and Trim-Away conditions. d, Quantification of aPKC cortical intensity (based on rabbit anti-aPKC signal) in outer cells of human embryos shown in c (n = 54 cells from 6 embryos). Data are presented as mean ± s.d. Two-tailed Student’s t-test, **P = 0.006. e, Immunofluorescence analysis of anti-mouse IgG secondary antibody to detect the electroporated mouse anti-aPKC primary antibody, mCherry, and DAPI nuclear staining in cow morula embryos in control and Trim-Away conditions at a pulse length of 5 or 7.5 ms (n = reported in Supplementary Table 10 for each condition). f, Immunofluorescence analysis of GATA3, YAP1, anti-mouse IgG secondary antibody to detect the electroporated mouse anti-aPKC primary antibody and DAPI nuclear staining in cow morula embryos in control and Trim-Away conditions. g, h, Quantification of YAP1 (g) and GATA3 (h) normalized fluorescence intensity in outer cells of cow embryos shown in f (n = 154 cells for YAP1 from 16 embryos, and n = 207 cells for GATA3 from 20 embryos). Data are presented as mean ± s.d. Two-tailed Student’s t-test, ns, not significant. Scale bars, 50 μm. i, Proposed model for human early lineage specification. EPI, epiblast; PrE, primitive endoderm; E-CAD, E-cadherin; β-CAT, β-catenin. More details about statistics and reproducibility are provided in Methods.
