Extended Data Fig. 2: Protein expression of TE-associated markers in mouse, cow and human morula embryos.
From: Initiation of a conserved trophectoderm program in human, cow and mouse embryos
a, b, Immunofluorescence analysis of F-actin, TEAD4 and Hoechst-33342 nuclear staining in human morula embryo. Yellow arrowheads point to a cell without TEAD4 expression. c, Percentage of TEAD4-positive and -negative cells in human morula embryos (n = 112 cells from 5 embryos). d, Quantification of TEAD4 normalized fluorescence intensity in human morula embryos (n = 63 cells from 5 embryos). Data are presented as mean ± s.d. Two-tailed Student’s t-test, ns, not significant. e, Immunofluorescence analysis of GATA3, E-cadherin, YAP1 and DAPI nuclear staining in mouse morula embryos. f, g, Quantification of YAP1 (f) and GATA3 (g) normalized fluorescence intensity in mouse embryos shown in e (n = 182 cells for YAP1 from 13 embryos and n = 139 cells for GATA3 from 9 embryos). Data are presented as mean ± s.d. Two-tailed Mann–Whitney U test, ****P < 0.0001. h, Immunofluorescence analysis using various secondary antibodies and DAPI nuclear staining (blue) in mouse morula embryos (n = 3). i, Time-course immunofluorescence analysis of GATA3, β-catenin, YAP1 and DAPI nuclear staining in cow embryos at different developmental stages: 8-cell (n = 5), late compaction (n = 5), morula (n = 10) and expanded blastocyst (n = 5). j, k, Quantification of YAP1 (j) and GATA3 (k) normalized fluorescence intensity in cow morula embryos shown in i (n = 97 cells from 10 embryos). Data are presented as mean ± s.d. Two-tailed Mann–Whitney U test, ****P < 0.0001.l, Immunofluorescence analysis of GATA3, GATA2 and DAPI nuclear staining in a human morula embryo (n = 3). m, Immunofluorescence analysis of GATA3, OCT4, GATA2 and DAPI nuclear staining in a human expanded blastocyst embryo (n = 3). Yellow arrowheads point to outer cells expressing GATA3, and cyan arrow points to an inner cell devoid of GATA3 expression. Scale bars, 25 μm (mouse embryos), 50 μm (cow and human embryos). More details about statistics and reproducibility are provided in Methods.
