Extended Data Fig. 3: WWTR1, KRT18 and GRHL2 expression in human morula embryos.
From: Initiation of a conserved trophectoderm program in human, cow and mouse embryos
a, Immunofluorescence analysis of YAP1, F-actin, WWTR1 and DAPI nuclear staining in a human morula embryo (n = 3). b, Immunofluorescence analysis of YAP1, Nanog, WWTR1 and DAPI nuclear staining in a human expanded blastocyst embryo (n = 3). Yellow arrowheads point to outer and TE cells co-expressing YAP1 and WWTR1. c, Immunofluorescence analysis of GATA3, KRT18 and Hoechst-33342 nuclear staining in a human expanded blastocyst embryo (n = 3). d, Genome browser view of the ATAC-seq signal at the GRHL2 locus. High-confidence peaks (FDR-corrected P < 0.001; MACS2 dynamic Poisson model) were used to identify transcription factor (TF) motifs. Representative binding motifs associated with the footprints are highlighted. The average expression of GRHL2 in GATA3-high- and GATA3-low-expressing cells at the morula is shown and the TPM units are indicated. e, Immunofluorescence analysis of GRHL2, F-actin, GATA3 and DAPI nuclear staining in human morula embryos (n = 3). Yellow arrowheads point to outer cells co-expressing GRHL2 and GATA3, and cyan arrows point to inner cells expressing GRHL2 only. f, Immunofluorescence analysis of GRHL2, OCT4, GATA3 and DAPI nuclear staining in human expanded and hatching blastocyst embryo (n = 3 each stage). Scale bars, 50 μm. More details about statistics and reproducibility are provided in Methods.
