Extended Data Fig. 5: SOX2 is an inner-cell-specific marker in mouse, but it is broadly expressed in cow and human morula embryos.
From: Initiation of a conserved trophectoderm program in human, cow and mouse embryos
a, Immunofluorescence analysis of SOX2, E-cadherin, GATA3 and DAPI nuclear staining in mouse morula embryos. b, Quantification of SOX2 normalized fluorescence intensity in mouse embryos shown in a (n = 93 cells from 7 embryos). Data are presented as mean ± s.d. Two-tailed Mann–Whitney U test, ****P < 0.0001. Yellow arrows mark outer cells expressing only GATA3. c, Time-course immunofluorescence analysis of SOX2, β-catenin, GATA3 and DAPI nuclear staining in cow embryos at 8-cell (n = 5), late compaction (n = 5), morula (n = 10) and expanded blastocyst (n = 5) stages. d, Immunofluorescence analysis of SOX2, β-catenin, GATA3 and DAPI nuclear staining in human 8-cell (n = 5) and late-compaction (n = 5) stage embryos. e, Quantification of SOX2 normalized fluorescence intensity in cow morula embryos shown in c (n = 136 cells from 10 embryos). Data are presented as mean ± s.d. Two-tailed Student’s t-test, ns, not significant. f, Quantification of SOX2 normalized fluorescence intensity in human morula embryos shown in Fig. 2b (n = 68 cells from 6 embryos). Data are presented as mean ± s.d. Two-tailed Student’s t-test, ns, not significant. g, Immunofluorescence analysis of SOX2, F-actin, GATA3 and DAPI nuclear staining in human morula and expanded blastocyst embryo (n = 3 each stage). The SOX2 antibody used in g in this figure is MAB2018 (R & D), whereas the one used in Fig. 2 is 14-9811-82 (Ebioscience); both show a consistent signal for SOX2. Yellow arrowheads point to outer cells co-expressing GATA3 and SOX2, and cyan arrow points to an inner cell showing SOX2 expression only. Scale bars, 25 μm (mouse embryos), 50 μm (cow and human embryos). More details about statistics and reproducibility are provided in Methods.
