Extended Data Fig. 6: Apical PAR complex and AMOT expression in mouse, cow and human morula embryos.
From: Initiation of a conserved trophectoderm program in human, cow and mouse embryos
a, Immunofluorescence analysis of aPKC, E-cadherin, AMOT and DAPI nuclear staining in mouse morula embryos (n = 10). b, Fluorescence intensity profile of aPKC and AMOT shown along the yellow arrows in the mouse morula embryo shown in a. a.u., arbitrary units. c, Immunofluorescence analysis of aPKC, E-cadherin and DAPI nuclear staining in mouse morula embryo (n = 10). d, Immunofluorescence analysis of aPKC, β-catenin, AMOT and DAPI nuclear staining in cow morula embryos (n = 10). e, Fluorescence intensity profile of aPKC and AMOT shown along the yellow arrows in the cow morula embryo shown in d. a.u., arbitrary units. f, Immunofluorescence analysis of aPKC and DAPI nuclear staining in cow morula embryo (n = 3). g, Immunofluorescence analysis of aPKC, E-cadherin and DAPI nuclear staining in human morula embryo (n = 3). The aPKC antibody used in c, f, g is LC-C354069 (LSBio), whereas the aPKC antibody used in Fig. 2c and in a, d, h, i is sc-17781 (Santa Cruz). Both antibodies show consistent and strong aPKC apical expression. h, Immunofluorescence analysis of PARD6B, F-actin, aPKC and DAPI nuclear staining in human morula embryo (n = 3). i, Immunofluorescence analysis of PARD6B, Nanog, aPKC and DAPI nuclear staining in human expanded blastocyst embryo (n = 3). Scale bars, 25 μm (mouse embryos), 50 μm (cow and human embryos). More details about statistics and reproducibility are provided in Methods.
