Extended Data Fig. 8: Eif2a cKIPvalb mice show no change in consolidation of contextual and auditory fear memory.
From: eIF2α controls memory consolidation via excitatory and somatostatin neurons
a, Diagram depicts the genotypes of mice used in the experiments. b, c, Immunofluorescent labelling of total and p-eIF2α in PVALB+ neurons shows reduced p-eIF2α levels in Eif2a cKIPvalb mice (t 11.86 = 3.5; n = 7, 7, points represent means per mouse). Two independent experiments showed similar results. d, Long-term contextual fear memory is not changed in Eif2a cKIPvalb mice (F 1, 34 = 0.85; n = 9, 10). e, Long-term auditory fear memory remains intact in Eif2a cKIPvalb mice (F 1, 32 = 0.124; n = 9, 9). f, In an open field test, both groups spent a similar amount of time in the outer and inner zones (F 1, 32 = 6.6 × 10−12; n = 9, 9). Representative heat-map of travelled path in an open field arena. g, h, Immunohistochemistry of puromycin in Eif2a cKIPvalb shows enhanced protein synthesis (t 10.67 = 5.85; n = 15, 8, points represent means per mouse). Two independent experiments showed similar results. Data are presented as mean ± s.e.m. in c–f, h. p-values by two-tailed unpaired t-test with Welch’s correction in c and h are indicated. Points represent individual mice unless stated otherwise. Scale bars: 20 μm.
