Extended Data Fig. 9: No change in L-LTP threshold in Eif2a cKIPvalb mice.
From: eIF2α controls memory consolidation via excitatory and somatostatin neurons
a–c, A 1 × HFS induced short-lasting E-LTP in Pvalb-Cre+ and Eif2a cKIPvalb hippocampal slices (E-LTP, F 1, 6 = 1.917; n = 7, 7). d, e, L-LTP induced by four tetanic trains at 100 Hz is similar in Pvalb-Cre+ and Eif2a cKIPvalb slices (e; L-LTP, t 7.542 = 0.6136; n = 6, 5). f, Diagram of experimental arrangement with whole cell recording of mEPSCs (in PVALB+) and mIPSCs (in excitatory neurons). g, Sample traces of mEPSCs. h, Cumulative distribution of mEPSC inter-event intervals (nmice = 13, 11, points represent group means). Inset shows similar mEPSC frequency in Pvalb-Cre+ and Eif2a cKIPvalb (t 15.41 = 0.84; n = 13, 11). i, Cumulative distribution of mEPSC amplitudes (nmice = 13, 11, points represent group means). Bar graph in inset shows reduced mEPSC amplitude in Eif2a cKIPvalb (t 20.34 = 3.35; n = 13, 11). j, Sample traces of mIPSCs. k, Cumulative distribution of mIPSC inter-event intervals (nmice = 10, 10, points represent group means). Inset shows similar mIPSC frequency (t 17.83 = 0.52; n = 10, 10). l, Cumulative distribution of mIPSC amplitudes (nmice = 10, 10, points represent group means). Inset, mIPSC amplitude is not changed between the groups (t 13.70 = 2.04; n = 10, 10). m, Diagram of experimental arrangement for recording intrinsic and firing properties from parvalbumin neuron. n, Resting membrane potential (t 17.55 = 0.79; n = 10, 10). o, Input resistance (t 17.92 = 0.38; n = 10, 10). p, The F/I gain relationship is similar in the Pvalb-Cre+ and Eif2a cKIPvalb mice (t 18 = 1.72; n = 10, 10). q, Representative traces obtained in response to 200 pA current injection in the parvalbumin-expressing interneurons from Pvalb-Cre+ and Eif2a cKIPvalb mice. r, Representative illustration of target area for the injection of AAV9-EF1α-DIO-EYFP-WPRE-hGH to label PVALB-expressing neurons in the dorsal hippocampus. Two independent experiments showed similar results. Data are presented as mean ± s.e.m. in a–e, h, i, k, l, n–p. p-values by Kolmogorov–Smirnov test in i and by two-tailed unpaired t-test with Welch’s correction in i(inset) are indicated. Points represent individual mice unless stated otherwise. Scale bars: 200 μm. Calibration: 0.3 mV, 5 ms.
