Extended Data Fig. 5: Analysis of excitatory neurons-specific translational landscape and proteomics from hippocampi of Eif2a cKICamk2a mice and following fear conditioning learning.
From: eIF2α controls memory consolidation via excitatory and somatostatin neurons
a, Pairwise Pearson correlation coefficient between log2 fold changes in translation efficiency comparing the genotype effect (naive Eif2a cKICamk2a vs. naive control) and learning effect (1 h fear conditioning control vs. naive control) using all translated mRNAs (11,805 transcripts). b, Pairwise Pearson correlation coefficient between log2 fold changes in protein levels comparing the genotype effect (naive Eif2a cKICamk2a vs. naive control) and learning effect (24 h fear conditioning control vs. naive control) using all CA1 expressed proteins (5,237 proteins). Pearson correlation coefficients were significant in both cases (P < 0.0001), dots marked red denote transcripts/proteins differentially translated/expressed. c, Functional enrichment in protein subset similarity differentially expressed between proteomes of the two genotypes (52 proteins), and between proteomes of treatment vs. naive mice from both genotypes (27 proteins). GO ontologies, BioPlanet pathways and Reactome pathways showing significant enrichment (FDR adjusted P < 0.1) are presented. Nodes represent proteins, edges represent pathway/ontology relatedness. Black edges are links found by String analysis and represent co-expression evidence. Blue nodes denote reduced level of expression; red nodes denote increased level of expression. * denote proteins differentially expressed following 24 h fear conditioning in both mouse genotypes, but not between naive mice.
