Extended Data Fig. 9: Optical inhibition of OT neurons perturbs the re-tuning of auditory cortical neurons.
From: Innate and plastic mechanisms for maternal behaviour in auditory cortex
a, Example in vitro current-clamp recording from pup-naive auditory cortex. The probability of evoking spikes in response to five extracellular stimulus pulses (ISI of 575 ms) was measured before and after an oxytocin (1 μM) wash. b, Left, evoked spike probability was significantly enhanced 15–30 min after the onset of oxytocin wash (n = 9 cells; P = 0.34 (stim 1), P = 0.01 (stim 2), P = 0.01 (stim 3), P = 0.001 (stim 4), P = 0.02 (stim 5)). Right, repetitive stimulation in the absence of oxytocin (ACSF, n = 7 cells) did not induce changes in spike probability (all stim, P > 0.99; one-way Friedman test with Dunn’s correction). Mean ± s.e.m. c, Example in vitro current-clamp recording from an oxytocin neuron containing halorhodopsin (eNpHR3.0). Photostimulation efficiently perturbed spiking for the duration of the light. d, Latency to retrieve on successful trials from Fig. 4e (CH+575sham (n = 32 trials) vs CH+575opto-OT (n = 20), P = 0.0003; unpaired two-tailed Mann–Whitney test). Median ± interquartile range. e, Temporal tuning curves from CH+575opto-OT virgins in Fig. 4f (n = 4 mice). Each coloured line represents the tuning curve for one virgin.
