Extended Data Fig. 6: The effects of Ubx affinity on morphology.
From: Dense and pleiotropic regulatory information in a developmental enhancer
a, b, Schematic output from NRLB15 shows predicted binding affinity for Exd::Ubx heterodimers across the E3N sequence, where black peaks are on the 5′ strand and red peaks respectively on the 3′ strand. Affinity plots are shown for Drosophila melanogaster (a) and Drosophila virilis (b). c, d, Drosophila cuticle preps for flies with WT E3N driving svb cDNA (c), or E3N with increased Ubx binding affinity driving svb cDNA (d). Trichomes were counted within a region of interest (teal box) defined by anatomic epithelial sensory cells (*). Arrows and brackets demarcate ectopic trichomes. e, Boxplots comparing trichome numbers in the A1 segment in the region of interest from panels (c) and (d) (n = 13, P < 0.02), see also Tsai et al., 201824. In box plots, centre line is mean, upper and lower limits are standard deviation and whiskers show 95% CIs. Scale bars, 25 μm each.
