Extended Data Fig. 1: Distribution of mutations in the E3N enhancer library.
From: Dense and pleiotropic regulatory information in a developmental enhancer
a, Mutant enhancer variants of E3N were created via degenerate PCR and integrated into the placZattB plasmid, which contains a minimalized core hsp70 promoter and the lacZ reporter gene. Plasmids were integrated into the Drosophila genome at the attP2 site. b, Pie chart depicting base-pair composition of the WT E3N enhancer. c, (Left) Histogram for all 749 mutants (dark red) is approximately normal with an average of 7 mutations per mutant. Magenta bars denote lines antibody stained (117 total), and blue lines indicate lines that were also Beta-Galactosidase stained (274 total). (Right) pie chart shows probability of mutation normalized to ATCG composition (see b). d, Manhattan plot shows the summation of all mutations within the E3N library. e, Unsmoothened “footprinting scores” from Fig. 1h. Scores plotted linearly over transcription factor binding motifs (colored and shaded regions) across the E3N genomic sequence.
