Extended Data Fig. 6: Multi-colour in situ hybridization for anatomical validation of transcriptomic cell types. | Nature

Extended Data Fig. 6: Multi-colour in situ hybridization for anatomical validation of transcriptomic cell types.

From: The cellular basis of distinct thirst modalities

Extended Data Fig. 6

a, Quantification of SFO Htr7- and Rxfp1-positive cells and their overlap in the SFO (n = 17 sections from 6 animals). Scale bar, 20 μm. Nuclei are visualized by DAPI staining (white). b, Quantification of Bmp3- and Rxfp1-positive cells and their overlap in the OVLT (n = 15 sections from 8 animals). Scale bar, 20 μm. c, Rxfp1-and Pdyn-positive cells co-express Fos under water deprived conditions. Representative images from 8, 3, 8 and 3 sections from 2 independent experiments for SFO RXFP1/FOS, OVLT RXFP1/FOS, SFO PDYN/FOS and OVLT PDYN/FOS stains respectively. Scale bar, 10 μm. d, Cell types labelled by Rxfp3 (SFO) and Cpne4 (OVLT) express Fos under osmotic thirst conditions (left). Cell types labelled by Htr7 (SFO) and Bmp3 (OVLT) express Fos under hypovolaemic thirst (right). Representative images from 2, 2, 3 and 2 sections from 2 independent experiments for SFO RXFP3/FOS, OVLT CPNE4/FOS, SFO HTR7/FOS and OVLT BMP3/FOS stains respectively. Scale bar, 10 μm.

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