Extended Data Fig. 2: Cryo-EM for GABAAR.
a, B-factor plots for three datasets using an X-FEG, the new energy filter with a slit width of 3eV (orange), 5eV (blue) and 10 eV (grey) and a Falcon-4 camera. b, Two orthogonal views of an electron tomogram for ice thickness measurement. Scale bar is 50 nm. c, Representative electron micrograph from the CFEG dataset. Scale bar is 30 nm. d, A selection of 2D class average images. e, Local resolution map. f, Fourier Shell Correlation (FSC) between the two independently refined half-maps. g, FSC between the model and the map calculated for the model refined against the full reconstruction (black); the model refined in the first half-map against that half-map (FSCwork; blue); and the model refined in the first half-map against the second half-map (FSCtest; dashed orange). Atomic models were refined including spatial frequencies up to 1.63 Å.
