Extended Data Fig. 3: Upk2- and Scn10a-cre expression and bladder weights.
From: PIEZO2 in sensory neurons and urothelial cells coordinates urination
a, Upk2-cre;Ai9 bladder tissue fixed, frozen and mounted to show tdTomato (red) throughout the urothelium, labelled with DAPI (blue). Expression was evaluated in two mice. b, Scn10a-cre;Ai9 bladder tissue fixed, frozen and mounted to show tdTomato (red) is not present. Expression was evaluated in two mice. Thin cryosections made neuronal endings difficult to visualize. Scale: 200 μm, applies to a and b. c, Scn10a-cre;Ai9 DRG tissue showing tdTomato (red) in the majority of neurons, and d, a cell backlabelled with CTB-Alexa 488 injected into bladder. e, Merge of c and d, DAPI in blue. 9/9 backlabelled bladder cells analysed from two mice were tdTomato positive. f, Quantification of freshly excised bladder weights from four Upk2-cre;Piezo2fl/fl knockout and wild-type littermates. Age-matched littermates were 10–11 months old, which could account for greater variability. g, Bladder weights from age-matched Scn10a-cre;Piezo2fl/fl knockout mice and wild-type littermates, 7–8 months old. Red lines indicate mean values.
