Extended Data Fig. 6: Simultaneous molecular and immunophenotypic profiling of samples from patients with AML.
From: Single-cell mutation analysis of clonal evolution in myeloid malignancies
a, UMAP plot of MSK54 with cells clustered by immunophenotype. Genotype (wild type = grey; DNMT3A = red; IDH2 = green; DNMT3A/IDH2 double mutant = blue) overlaid onto each cell. b, UMAP from a with protein expression (high expression = red; low expression = blue) for each of the six antibody targets (CD3, CD11b, CD34, CD38, CD45RA, CD90) overlaid onto each cell. Relative protein expression is normalized across individual samples by CLR. c, Immunophenotype changes based on co-occurring mutations in clones. Heat map of normalized protein expression of CD34 (top panel) and CD11b (bottom panel) in DNMT3A and IDH1/2 single-mutant clones versus DNMT3A and IDH1/2 mutant clones with co-occurring NRAS or FLT3 mutations. High protein expression depicted in red and low protein expression depicted in blue.
