Extended Data Fig. 8: Neighbourhood analysis of all single-cell DNA+Protein AML samples.
From: Single-cell mutation analysis of clonal evolution in myeloid malignancies
a, Divergences in cell-surface protein expression of CD34, CD38, CD11b, and CD45RA determined by presence of signalling effector mutation. Density plots of cells from MSK71 (further detailed in Figure 4c–f and Extended Data Figure 7) of DNMT3A mutant cells (yellow = single-mutant) with co-occurring FLT3 (black), KRAS (orange), or NRAS (light blue) mutations. Concentration of cells with a given immunophenotype depicted by the density of lines. b, UMAP plot of samples (n = 17) analysed by DNA+Protein single-cell sequencing with cells clustered by cell-surface protein expression of 6 antibody targets (CD3, CD11b, CD34, CD38, CD45RA, CD90). Cells from the same sample are denoted with same colour. c, Neighbourhood analysis of all samples from UMAP from b with communities of cells identified by neighbourhood analysis in overlaid colours.
