Extended Data Fig. 9: Clone- and gene- specific alterations to cell-surface protein expression and community representation in AML samples.
From: Single-cell mutation analysis of clonal evolution in myeloid malignancies
a, Column normalized heat map of cell-surface protein expression for each community identified in phenoGraph analysis on UMAP from Extended Data Figure 8b, c. Expression is depicted by colour with blue being low expression and red annotating high expression. b, Community representation changes across all samples (n = 14) in the wild type, the dominant clone, and all subclones. The fraction of each sample within each community is shown with communities depicted by corresponding colour. Samples without communities shown for wild-type cells were found to not have any wild-type cells present in analysis. Changes in immunophenotype due to community representation changes for samples MSK94 (P ≤ 9.95 × 10−3) and MSK130 (P ≤ 2.45 × 10−8) are highlighted in c. A two proportions z-test for each sample was used to determine statistical significance between dominant clone communities and communities present in subclone ***P < 0.001. c, Cell-surface protein expression of CD11b, CD34, and CD38 between dominant clone (red) and subclones (black) in an FLT3-ITD mutant sample (MSK130; right panel; n = 2274 total cells) and JAK2 mutant sample (MSK94; left panel; n = 6012 total cells). Each error bar represents a distinct community that is significantly expanded or contracted, (error bar indicates ± standard error of measure, from the mean expression of indicated protein in a given community). A Student’s t-test was used to determine statistical significance *P < 0.1; **P < 0.01; ***P < 0.001.
