Extended Data Fig. 7: Mutational patterns in germ-free/Oligo-MM¹² public clonotypes.

a, Dendrograms showing the sequence relationships between V_H1–47 and V_H1–12 clones in different mice. All clones with up to two-amino-acid differences from the public-clonotype CDR_H3 motifs are included. b, Heat maps showing the frequency of amino-acid replacements along the V_H1–47 and VH_H1–12 families in germ-free (blue) and Oligo-MM¹² (green) mice, using the same data as in Fig. 4b. Only mice with more than two cells within the specified clone were included in the analysis. The number of cells analysed per mouse is indicated at the top of each column. Only those amino acids mutated in at least three (V_H1–47) or two (V_H1–12) mice are listed on the left, using Immunogenetics (IMGT; http://www.imgt.org) numbering; to the right, the most frequent amino-acid replacement in each mouse is given. Arrows indicate recurrent amino-acid mutations found in five of six mice (V_H1–47) or three of three mice (V_H1–12).