Extended Data Fig. 1: Amplification plot for sample pools.
From: A pooled testing strategy for identifying SARS-CoV-2 at low prevalence
Each of the 64 sample pools described in the text tests negative for SARS-CoV-2: the RT–PCR fluorescence curves show below-threshold net fluorescence values. By contrast, for both target genes of the positive control, the fluorescence curves cross the threshold after 32 PCR cycles. ΔRn denotes the difference between the fluorescence signal generated by a sample and a baseline signal. The yellow curves reaching ΔRn ≈ 2,000,000 and 1,750,000 represent the positive control for the N and orf1ab genes, respectively. The other yellow and orange curves represent internal controls.
