Extended Data Fig. 6: HSP110 role in disaggregation is independent of ATPase cycle.
From: Molecular dissection of amyloid disaggregation by human HSP70
a, Disaggregation of α-syn fibrils by the HSP70 machinery (DNAJB1, HSP70) in the presence of wild-type HSP110 (black), ATPase-deficient HSP110 (light green, D7S)23 or NEF-deficient (ΔNEF) HSP110 (green, A279A/N280A/N619Y/E622A)23. b, Dissociation of HSP70AF488 from amyloid fibrils triggered by the addition of 0.1× wild-type HSP110 (black), ATPase deficient HSP110 (light green) or NEF-deficient HSP110 (green) in the presence of ATP. c, Steady-state ATPase rates of HSP70 in the presence of 1× DNAJB1 and 2× BAG1 (red), 2× H6-sumo-BAG1 (orange) or 2× H6-sumo-BAG1 with 2.5× anti-H6 (green), data are mean ± s.e.m. d, ThT fluorescence-based disaggregation assay of α-syn fibrils with the full (untagged) HSP70 machinery (DNAJB1, HSP70, HSP110) in the absence (black) or presence of 1.25× anti-H6 (red). Reaction conditions and number of replicates are specified in Extended Data Table 2.
