Extended Data Fig. 10: Multiunit activity (MUA) and ripple- and theta-event density functions (EDF) for the sessions with recordings from two PGO-related structures.
From: Coupling of hippocampal theta and ripples with pontogeniculooccipital waves
The events included in this figure were selected as “propagating”, namely as events in the first PGO-related structure, which induced a suprathreshold response in a second structure. The sign of the Phase-Locking Value (PLV) implying the that second structure is following the first did not change the profile of responses shown here. a, Pontine MUA and Hippocampal Event Density Functions (EDFs) for the single PBn-LGN-HC session (left group of plots), and for LGN-VCx-HC sessions, where LGN was the first and Cortex the second PGO-related anatomical structure (right group of plots). Experimental sessions and groups associated with each group of plots are indicated at the top. Type-I PGO events, as shown in main text figures, are strongly associated with occurrence of hippocampal ripples, while the Type-II PGO events are associated with theta-bouts. Shaded areas indicate SEM. b, Same as a, but in this case events were simply detected in the first PGO-associated structure and were subsequently clustered in an unsupervised fashion using the Growing Neural Gas-based clustering algorithm (Supplementary Methods). Grey areas correspond to SEM estimated from the data of all groups (spont & spont1) across experimental sessions. c, Percentage of propagating events (PGO waves) that were predicted by unsupervised clustering, assuming the detection of PGO waves using a second PGO-associated structure as the ground truth (left subpanel). For most sessions, the events are consistently predicted as PGO waves, corresponding to more than 80% of the ground-truth events. The percentage of each PGO wave type (right subpanel) shows that there are characteristic differences and variability across sessions, probably reflecting the state of the animal during individual preparations. Sessions in which hippocampus and LGN were recorded (animal e10) yielded virtually identical results.
