Extended Data Fig. 2: Selectively expressed RNA markers of human immune cell types from bulk mRNA sequencing of FACS-purified immune cells.
From: A molecular cell atlas of the human lung from single-cell RNA sequencing
a, Heat map of RNA expression of the most selectively-expressed genes from bulk mRNA sequencing of the indicated FACS-sorted immune populations (Supplementary Table 3). This dataset provided RNA markers for human immune cell populations that have been classically defined by their cell surface markers. b, Heat map of pairwise Pearson correlation scores between the average expression profiles of the immune cell types indicated that were obtained from bulk mRNA sequencing (BulkSeq, a) to the average scRNA-seq profiles of human blood immune cells in the SS2 dataset annotated by canonical markers and enriched RNA markers from the bulk RNA-seq analysis. The highest correlation in overall gene expression (white dot) of each annotated immune cell cluster in the SS2 dataset (columns) was to the bulk RNA-seq of the same FACS-purified immune population (rows), supporting the scRNA-seq immune cluster annotations (red squares). Cell numbers are in Supplementary Table 2. For more details on statistics and reproducibility, see Methods.
