Extended Data Fig. 9: The effect of NrCAM on excitatory synapse formation and function in vivo.
From: Chemico-genetic discovery of astrocytic control of inhibition in vivo
a, Images of postsynapse PSD95 and presynapse VGLUT1 within NrCAM-deletion astrocytes in L1 of the visual cortex. High magnification images (bottom) correspond to boxes (above). b, Quantification of average number of excitatory synaptic colocalized puncta within astrocyte territories. n = 15 cells per each condition from 3 mice. c, mEPSC traces from L2/3 pyramidal neurons following astrocyte control empty sgRNA or NrCAM sgRNA expression. d–g, Quantification of mEPSC amplitude (d, e, Cont = 16, NrCAM sgRNA = 14 cells from 4 mice) and frequency (f, g, Cont = 14, NrCAM sgRNA = 17 cells from each of 4 mice). At least n = 3 biological repeats. Student’s t-test (paired, P < 0.05). Data represent means ± s.e.m.
