Extended Data Fig. 8: Level of tricarboxylic acid metabolites and some factors.
From: Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis
a, Proposed link between cholesterol biosynthetic pathway and succinate. b, Levels of the metabolites in liver. The male WT and L-Usp20−/− littermates (n = 4 per group) were fasted for 12 h and then refed for 6 h. The metabolites were measured by liquid chromatography-tandem mass spectrometry (LC-MS/MS). c, Changes in the metabolites associated with the tricarboxylic acid cycle in liver. The male WT and L-Usp20−/− littermates (n = 4 per group) were fasted for 12 h and then refed for 6 h. Liver metabolites were measured by LC-MS/MS. The fold change was calculated through dividing the L-Usp20−/− mice value by the WT mice mean value. *P < 0.05 for the level of statistical significance (unpaired two-tailed Student’s t-test) between WT and L-Usp20−/− mice. d–l, The epinephrine (d), norepinephrine (e), triiodothyronine (T3) (f), thyroxine (T4) (g), secretin (h), thyroid-stimulating hormone (TSH) (i), FGF21 (j), ALT (k) and AST (l) of L-Usp20−/− and WT mice (n = 6 per group). The data were analysed by unpaired two-tailed Student’s t-test (b, d–l). The values present as mean ± SEM.
