Extended Data Fig. 8: PDGFRα⁺PDGFRβ⁺ cells in kidney fibrosis.

a, Scaled expression of the top 10 genes by specificity in each of the mesenchymal cell clusters depicted in Fig. 3d. Each 100 cells are averaged in one column. b, Cell doublet score (Methods) of mouse PDGFRα⁺PDGFRβ⁺ dataset per cell cluster. c, Violin plot of COL15α1 expression per cell cluster. Only mesenchymal cells are shown. Bonferroni corrected P values based on a two-sided Wilcoxon rank sum test in Supplementary Data 4. d, UMAP embedding of MEG3 as in Fig. 2a and multiplex in-situ staining of MEG3 on human kidney tissue. Scale bars, 30 μm (d1), 40 μm (d2+3). e, Representative image of multiplex RNA ISH for PDGFRA, PDGFRB and MEG3 in n = 34 human kidneys (patient data in Supplementary Table 2). MEG3 colocalizes with PDGFRA and PDGFRB. Scale bar, 10 μm. f, Percentage of MEG3⁻ cells out of PDGFRα⁺PDGFRβ⁺ double-positive cells, quantified from RNA ISH. n = 34. Tukey box and whisker plot. g, Expression of select genes on the UMAP embedding from Fig. 3j. For details on statistics and reproducibility, see Methods.