Extended Data Fig. 4: PDGFRβ+ cell enrichment.
From: Decoding myofibroblast origins in human kidney fibrosis
a, Patient samples (n = 8) visualized on the UMAP from Fig. 2a. Different cell clusters are indicated by different colours. Stratification of single cells according to patient clinical parameters. b, c, Expression of select genes on the same UMAP embedding from a. d, Scaled gene expression of the top 10 genes in each cell type/state cluster. Gene ranking per cell cluster was determined by genesorteR. e, Correlation between cell clusters identified in CD10− data (Fig. 1, columns) and PDGFRβ+ data (Fig. 2, rows). f, ECM score stratified by four main cell types in PDGFRβ+ data. g, ECM score stratified by main mesenchymal cell types. h, ECM score stratified by five epithelial cells clusters. i, ECM score visualized on the UMAP embedding from a. j, Doublet score (Methods) for human PDGFRβ+ cells. k, Representative image of combined immunofluorescent and multiplex RNA ISH of LTA (proximal tubular marker), COL1A1 and PDGFRB. Note COL1A1 and PDGFRB expression in LTA+ tubular cells (j, arrows). l, Representative image of combined immunofluorescent and multiplex RNA ISH of CD68 (macrophage marker), COL1A1 and PDGFRB. m, Representative image of multiplex RNA ISH of PECAM1, COL1A1 and PDGFRB. Scale bars, 50 μm.
