Extended Data Fig. 7: Effects of manipulating the activity of α’/β’ subset specific neurons on long-term memory.
From: Availability of food determines the need for sleep in memory consolidation
(a) and (b) Neurotransmission from α’/β’m neurons (UAS-shibirets1/R26E01 and UAS-shibirets1/MB370B) is dispensable for long-term memory in fed flies (n ≥ 6). Temperature controls are depicted in (b) (n ≥ 6) (one-factor ANOVA with Tukey post hoc test). (c) and (d) Blocking the activity of α’/β’ap neurons (UAS-shibirets1/R35B12 and UAS-shibirets1/VT50658) for 4 h after conditioning in starved flies has no effect on long-term memory (n = 6). Long-term memory in experimental and control flies at the permissive temperature, 25°C, is shown in (d) (n = 6) (one-factor ANOVA with Tukey post hoc test). (e) shibirets does not affect memory in flies maintained under starvation conditions at the permissive temperature (one-factor ANOVA with Tukey post hoc test; n = 6). Controls related to Fig. 2(a). (f) shibirets has no effect on memory in flies maintained on food at the permissive temperature (one-factor ANOVA with Tukey post hoc test; n ≥ 6). Controls related to Fig. 2(b). (g) Hyperactivation of α’/β’ap neurons (UAS-TrpA1/R35B12) for 4 h post-training does not affect long-term memory formation in starved flies (one-factor ANOVA with Tukey post hoc test; n = 6). (h) Memory was not affected in UAS-TrpA1/R35B12 flies at permissive settings (one-factor ANOVA with Tukey post hoc test; n = 6). Data are represented as mean ± s.e.m. Each data point in a memory experiment represents a group of flies. Precise ‘n’ and ‘p’ values are in the Source Data.
