Extended Data Fig. 1: Sleep increases in flies fed after appetitive training.
From: Availability of food determines the need for sleep in memory consolidation
(a) Flies starved for 6 h before training show no difference in sleep between trained and untrained groups. However, moving trained flies into sucrose tubes post-training resulted in a significant increase in sleep compared to untrained controls despite only 6 h of pre-training starvation. Sleep was quantified for the ZT8-12 interval (two-sided t-tests were performed for each condition to compare trained and untrained groups, followed by Bonferroni correction; n = 32). (b) and (c) Training increases sleep bout length in fed flies but not in starved flies. Flies were trained after 18 h (b) and 6 h (c) starvation (two-sided Mann–Whitney U-tests were performed for each condition to compare trained and untrained groups; n = 32). Data are represented as mean ± s.e.m. Each data point depicts a single fly. Precise ‘n’ and ‘p’ values are in the Source Data. ***P < 0.001; **P < 0.01; *P < 0.05.
