Table 2 Animal testing systems under development

From: The NIH Somatic Cell Genome Editing program

Organism

Editing events detected

Primary readout

Secondary readout

Editors

PIsa

Mouse

NHEJ, HDR, off-target cutting

Fluorescent signal in situ

Luciferase

SpyCas9, SauCas9, Cas12a

J. D. Heaney, M. E. Dickinson, W. R. Lagor

Mouse

NHEJ, HDR, base editing, PNA

Fluorescent signal in situ

Luciferase, NaI symporter

SpyCas9, SauCas9, Cas12a, Nme2Cas9, CjeCas9, ABE, CBE, PNA

S. A. Murray, C. M. Lutz

Pig

NHEJ, HDR

Fluorescent signal

NaI symporter

SpyCas9, SauCas9, Cas12a, ABE

D. F. Carlson; K. D. Wells, R.S. Prather

Macaque

NHEJ, HDR, C base editing

Fluorescent signal

Luciferase

SpyCas9, SauCas9, Cas12a, CBE

J. D. Hennebold; A. F. Tarantal, D. J. Segal

Marmoset

NHEJ

Akaluciferase

Fluorescence

SpyCas9, SauCas9, Nme2Cas9, Cas12a, ABE

G. Feng; A. F. Tarantal, D. J. Segal

  1. NaI, sodium iodide.
  2. aThe lead PI of the project is listed first. Additional PIs follow, listed alphabetically by last name. Reporter Development and Testing Center teams are separated by semicolons.
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