Extended Data Fig. 4: The architecture of myelopoiesis is similar in different sternum sections.

a, Observed distributions of distances for individual sternum sections (Ob), pooled values, or random (Rd) simulations for the data shown in Extended Data Fig. 2b. For distance to GMPs, n = 25, 22, 19, 20 GMPs per sternum section from a total of 4 sternum sections in 4 mice; n = 11, 12, 11, 10 MDPs per sternum section from a total of 4 sternum sections in 4 mice; n = 40, 37, 42, 46 MOPs per sternum section from a total of 4 sternum sections in 4 mice; n = 19, 21, 16, 26 GPs per sternum section from a total of 4 sternum sections in 4 mice. For distance to MDPs, n = 25, 22, 19, 20 GMPs per sternum section from a total of 4 sternum sections in 4 mice; n = 13, 14, 11, 9, 7, 14, 14, 14, 9, 11, 12, 10, 10, 11, 13, 11 MDPs per sternum section from a total of 16 sternum sections in 16 mice; n = 40, 37, 42, 46 MOPs per sternum section from a total of 4 sternum sections in 4 mice; n = 22, 24, 23, 28, 23, 16, 24, 18, 22, 21, 17, 26, 25, 20 GPs per sternum section from a total of 14 sternum sections in 14 mice. For distance to MOPs, n = 25, 22, 19, 20 GMPs per sternum section from a total of 4 sternum sections in 4 mice; n = 9, 11, 12, 10, 11, 13, 11, 10, 14, 11, 13, 10, 9, 11, 10, 14 MDPs per sternum section from a total of 16 sternum sections in 16 mice; n = 39, 49, 38, 33, 44, 36, 37, 44 MOPs per sternum section from a total of 8 sternum sections in 8 mice; n = 22, 24, 28, 23, 16, 24, 18, 22, 21, 17, 26, 25, 20 GPs per sternum section from a total of 13 sternum sections in 13 mice. For distance to GPs, n = 25, 22, 19, 20 GMPs per sternum section from a total of 4 sternum sections in 4 mice; n = 15, 12, 11, 10, 15, 9, 12, 11, 12, 11, 13, 10, 9, 11, 10, 15 MDPs per sternum section from a total of 16 sternum sections in 16 mice; n = 40, 37, 42, 46, 39, 39, 49, 53, 38, 34 MOPs per sternum section from a total of 10 sternum sections in 10 mice; n = 24, 23, 23, 28, 29, 22, 26, 17, 26 GPs per sternum section from a total of 9 sternum sections in 9 mice. b, c, As for a, but corresponding to the data shown in Fig. 2c, i. n = 24, 23, 28 GPs per sternum section from a total of 3 sternum sections in 3 mice. For MDPs to Ly6C^hi and Ly6C^lo monocytes, n = 11, 14, 8, 9, 9, 16 MDPs per sternum section from a total of 6 sternum sections in 4 mice. For MDPs to cDCs, n = 13, 14, 12, 9, 16, 15, 15, 10, 10, 10, 15 MDPs per sternum section from a total of 11 sternum sections in 6 mice. d, e, Throughout the paper, we have indistinctly used coronal and sagittal sternum sections. The maps (d) show granulopoiesis in coronal and sagittal sternum sections shown or analysed in Fig. 2a or Fig. 2c; the histograms show (e) observed and random distributions of distances for the indicated cells in each section or the pooled data for each type of section. n = 23 and 28 GPs in coronal sections and n = 24 and 23 GPs in sagittal sections. Scale bar, 200 μm. f, g, Representative maps showing the differentiation of monocytes and dendritic cells (f), comparing coronal and sagittal sternum sections with those shown or analysed in Fig. 2a, i; and observed and random distributions (g) of distances for the indicated cells in coronal or sagittal sections. n = 11 or 10 MDPs, n = 390 or 334 Ly6C^lo monocytes, and n = 218 or 258 cDCs in coronal sections; n = 9 or 10 MDPs, n = 419 or 380 Ly6C^lo monocytes, and n = 183 or 159 cDCs in sagittal sections. Scale bar, 200 μm. Statistical differences were calculated using two-tailed Student’s t-tests ; P values are shown.

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