Extended Data Fig. 7: Characterization of NINJ1 extracellular α-helix domain.
From: NINJ1 mediates plasma membrane rupture during lytic cell death
a, Computational model of the NINJ1 extracellular domain. Grey, hydrophobic residues; blue, negatively charged residues; light blue, polar residues; red, positively charged residues. b, Immunoblot of Flag–NINJ1 in HEK293T cells from Fig. 4e; representative of two independent experiments. c, Liposome cargo release by the NINJ1 α-helix region peptide, its sequence scrambled variant, or Melittin (α-helical bee venom peptide). Data are means (bars) of four individual replicates (circles). P value was calculated using a two-tailed unpaired Student’s t-test. d, Release of DD-150 in live-cell imaging of BMDMs after stimulation by nigericin in the presence of 20 μg ml−1 of indicated monoclonal antibodies. Data are means (circles) ± s.d. (shaded area) of four individual replicates. mAb, monoclonal antibody. For gel source data, see Supplementary Fig. 1.
