Extended Data Fig. 4: Phylogenetic tree and amino acid sequence alignment of NINJ1 and NINJ2.
From: NINJ1 mediates plasma membrane rupture during lytic cell death
a, Immunoblot of Flag–NINJ1 from Fig. 4a; representative of two independent experiments. b, LDH release from Flag–NINJ1 transfected HEK293T cells. Data are means (bars) of at least three individual replicates (circles) c, Cytotoxicity of non-tagged human and mouse NINJ1, NINJ2, and dNINJ-A, B, C in HEK293T cells. Data are means (bars) of at least four individual replicates (circles). d, Phylogenetic tree of NINJ1 and NINJ2. Numbers represent standardized distance scores (number of amino acid substitutions per length of the alignment). e, Multiple alignment of NINJ1 and NINJ2 amino acid sequences with the extracellular α-helix domain predicted by JPred highlighted in blue. f, Multiple alignment of NINJ1 amino acid sequences with the extracellular α-helix domain predicted by JPred. g, Circular dichroism spectra of NINJ1 α-helix region peptide that shows characteristic α-helix pattern (two dips at 208 nm and 222 nm). h, Immunoblot of Flag–NINJ1 from Fig. 4b; representative of two independent experiments. For gel source data, see Supplementary Fig. 1.
