Extended Data Fig. 9: Similarity of temporal expression profiles indicates associations in time.

Bottom, examples of temporal expression profiles for single-cell protein (blue) and RNA expression (orange). Line, moving mean; darker shade, 25th to 75th percentile range; lighter shade, 10th to 90th percentile range; points, individual cell data. The compartment for which the protein abundance was measured is denoted above: nuc, nucleus; cyto, cytoplasm; cell, entire cell. The proteins of interest are shown in green, and microtubules are shown in red. Similar temporal profiles between proteins indicate compartmentalization and association in time. For the G1 group, well-known CCD proteins such as ORC6, which is required for cell entry into S phase⁸⁷, and MCM10, which is required for DNA replication⁸⁸, were found to have similar patterns to the novel CCD protein ZNF32, whose overexpression has been associated with a shorter survival time in lung adenocarcinoma cells⁸⁹. The group that has peak expression at the end of G1 includes known proteins such as CCNE1, along with the novel CCD protein DUSP19 (Fig. 3), a phosphatase whose depletion results in increased mitotic defects⁹⁰. The G2 group includes known proteins, such as PC (Fig. 3), CCNB1, AURKB (Extended Data Fig. 2c, d) and BUB1B. Novel CCD proteins in this group include the phosphatase DUSP18 (Fig. 3); the transcription factor NFAT5 (Fig. 1F); a retrograde intraflagellar transport (IFT) protein TTC21B (Fig. 3), which has been implicated in several ciliopathies and may be implicated in additional roles beyond the primary cilium⁹¹; the oestrogen-sulfating enzyme PAPSS1 (Fig. 3), for which overexpression has been reported to affect proliferation⁹²; the methyltransferase N6AMT1 (Fig. 3); the uncharacterized protein PHLDB1 (Fig. 3); the enzyme DPH2; and transcription factor FLI1 (Fig. 3). Scale bar, 10 μm.