Extended Data Fig. 3: Flow cytometry of FUCCI cells.
From: Spatiotemporal dissection of the cell cycle with single-cell proteogenomics
Gating strategy used to sort U2OS FUCCI cells into three clusters representing three distinct segments of the cell cycle. This strategy distinguished the cells expressing the red tag (CDT1, G1) from the cells expressing the green tag (GMNN, S and G2). Cells that expressed both markers simultaneously, marking the S-transition, were clustered in a third group. The RNA velocities calculated for these cells (Fig. 2c, Extended Data Fig. 7a, b) validate this sorting strategy, as a cell cycle reflecting interphase progression is clearly observed.
