Extended Data Fig. 6: Single-cell RNA-seq of CD3⁺ T cells from livers of patients with steatosis or NASH.

a, UMAP plot visualization of hepatic CD3⁺ T cell clusters detected in livers from patients with steatosis (n = 4) or NASH (NAS ≥ 5) (n = 3). b, UMAP plot visualization of hepatic CD3⁺ T cell clusters and of selected genes detected in patients with NASH (NAS ≥ 5) (n = 3). c, Frequencies of CXCR6⁺ and PDCD1⁺ cells in CD8 T cells in the RGS1⁺, PLCG2⁺ and ITGA1⁺ cluster in livers from patients with steatosis or NASH. d, Differentially expressed genes in the CD8 RGS1⁺ cluster between livers of patients with steatosis or NASH. e, Flow cytometry analysis of FOXO1 expression in CD69⁺ CD8 T cells from livers of unaffected individuals (n = 3) and livers of patients with NASH (n = 5). P = 0.0088, two-tailed t-test, data are mean ± s.e.m. f, UMAP plot visualization of hepatic CD3⁺ T cell clusters detected in patients with NASH from fat tissue (n = 2). g, Jaccard similarity score between tissue-resident CD8 T cell populations in fat and liver of patients with NASH (n = 2). h, Correlation of mouse RNA-seq dataset from CXCR6⁺ compared to CXCR6⁻ CD8 T cells in mice fed a CD-HFD with human single-cell RNA-seq dataset from the CD8 GNLY⁺FGFBP2⁺ cluster. The solid line is the linear regression, and the grey area shows the 95% confidence intervals. Coefficient of determination (R²) and statistical significance (P value) were determined using Spearman’s correlation.

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