Extended Data Fig. 7: Anti-CD122 application in NASH mice leads to depletion of CXCR6+GzmB+ CD8 T cells and amelioration of liver damage.
From: Auto-aggressive CXCR6+ CD8 T cells cause liver immune pathology in NASH
a, CD122 expression on splenic CD44+ CD8 T cells after stimulation with IL-15 (10 ng ml−1) for 24 h (n = 5). b, CD122 expression on hepatic CXCR6+ CD8 T cells (n = 8). Two independent experiments. c–e, Frequencies and cell numbers of CXCR6+PD1+ and CXCR6+GzmB+ CD8 T cells after anti-CD122-treatment for 2 weeks in NASH mice (fed CD-HFD for 12 months) (CD-HFD + immunoglobulin (Ig), n = 6; CD-HFD + anti-CD122, n = 6). f, sALT in NASH mice from e before and after anti-CD122 treatment for 2 weeks. Unpaired two-tailed t-test of sALT values between groups before treatment, P = 0.7137. g–k, Numbers of CX3CR1 CD8 T cells, CD44+ CD4 T cells, natural killer T cells and natural killer (NK) cells in the liver after anti-CD122 antibody application from mice in e. l, m, No change in phenotype of natural killer cells in NASH mice. Dot plots are representative of ≥5 mice. Exact P values (a, b, e–m) are presented in Source Data. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. One-way ANOVA with Tukey’s multiple comparison test (e, g–k) and paired (a, f) and unpaired (b, l, m) two-tailed t-test. In a, b, e–m, data are mean ± s.e.m., error is reported as s.d.
