Extended Data Fig. 3: Transcriptome analysis of hepatic CXCR6+ CD8 T cells from NASH mice.
From: Auto-aggressive CXCR6+ CD8 T cells cause liver immune pathology in NASH
a, Volcano plot showing differentially expressed genes between CXCR6+ CD8 T cells from NASH mice (fed a CD-HFD) compared to healthy mice (fed a normal diet). b, GSEA for distinct pathways from a. c, Flow cytometry analysis of protein levels of transcription factors in hepatic CXCR6+ and CXCR6− CD8 T cells from healthy and NASH mice (n ≥ 5). d, Binding analysis for regulation of transcription (BART) to predict transcription-factor activity in hepatic CXCR6+ compared to CXCR6− CD8 T cells in NASH mice. e, Transcription-factor network analysis created from differentially expressed genes (a) and BART. f, GSEA for transcription-factor-dependent genes from a. g–i, Flow cytometry analysis of FOXO1 expression in hepatic CXCR6+ and CXCR6− CD8 T cells (g, h) or CD4 T cells (i) from mice fed a normal diet and NASH mice (n ≥ 6). j, k, Image-stream analysis of expression and localization of FOXO1 in different T cell populations from spleen and liver of wild-type mice with representative images. Tn, naive T cells; Tcm, central memory T cells; Tem, effector memory T cells. l, Similarity score to determine nuclear localization of FOXO1 and surface localization of CD44 (n = 5). m, n, FOXO1 expression in hepatic CD44+ CD8 T cells in mice fed a Western diet (n = 5) and ob/− or ob/ob mice (n ≥ 4). PI, propidium iodide. Exact P and n values (c, g-i) are presented in Source Data. *P < 0.05, **P < 0.01, ****P < 0.0001. One-way ANOVA (k, l), two-way ANOVA with Tukey’s multiple comparison test (c, i) and unpaired two-tailed t-test (h, m, n). In c, h, i, k–n, data are mean ± s.e.m., errors are shown as s.d.
