Extended Data Fig. 7: FASN is dispensable for the function of Treg cells in steady state and autoimmunity.
From: Lipid signalling enforces functional specialization of Treg cells in tumours
a, Expression of Hmgcr mRNA in Treg cells from Foxp3CreScap+/fl and Foxp3CreScapfl/fl mice under steady state (n = 5 samples per genotype). b, Expression of Hmgcr mRNA in Treg cells from Foxp3CreHmgcr+/+ (n = 4), Foxp3CreHmgcr+/fl (n = 6) and Foxp3CreHmgcrfl/fl (n = 4) mice under steady state. c, Analysis of T cell homeostasis in Foxp3CreHmgcr+/+ and Foxp3CreHmgcr+/fl mice (6–10 weeks; n = 3 mice per genotype): frequency of CD44hiCD62Llo cells amongst conventional Foxp3–CD4+ (left) and CD8+ T cells (right) from spleen and PLNs. d, Expression of Fasn mRNA in Treg, naive CD4+ and CD8+ T cells from Foxp3CreFasn+/fl and Foxp3CreFasnfl/fl mice under steady state (n = 3 samples per genotype). e–i, Analysis of spleen and PLNs from Foxp3CreFasn+/+ or Foxp3CreFasn+/fl control and Foxp3CreFasnfl/fl mice (8–12 weeks) under steady state (n = 4 mice in each group). e, f, Numbers of Foxp3+CD4+ Treg cells (e) and conventional Foxp3–CD4+ (f, left) and CD8+ (f, right) T cells. g, Frequencies of CD44hiCD62Llo cells amongst conventional Foxp3–CD4+ (left) and CD8+ (right) T cells. h, Frequencies of IFNγ+ (left), IL-4+ (middle) and IL-17+ (right) cells amongst conventional Foxp3–CD4+ T cells. i, Frequency of IFNγ+ cells amongst CD8+ T cells. j, Foxp3CreFasn+/+ or Foxp3CreFasn+/fl control (n = 5) and Foxp3CreFasnfl/fl (n = 6) mice were immunized with MOG, and the EAE disease score was measured every day until day 24. k, Mice as in j were euthanized in the recovery phase (day 24) of EAE for histological analysis of clinical disease. Left, haematoxylin-and-eosin staining of the spinal cord, and right, histological scores of the indicated regions of the brain and spinal cord (n = 5, Foxp3CreFasn+/+ or Foxp3CreFasn+/fl control mice; n = 6, Foxp3CreFasnfl/fl mice). ***P < 0.001; two-tailed unpaired Student’s t-test (a, c–i, k), one-way ANOVA (b), or two-way ANOVA (j). Data are means ± s.e.m. in a–k. Data are compiled from two (e–i) independent experiments.
