Extended Data Fig. 2: SCAP is dispensable for T_reg cells to maintain immune homeostasis under steady state.

a–d, Analyses of Foxp3^CreScap^+/+ or Foxp3^CreScap^+/fl (these mice are phenotypically indistinguishable) control and Foxp3^CreScap^fl/fl mice (12–18 weeks) under steady state (n = 4 mice in each group). a, Cellularity of conventional Foxp3^–CD4⁺ and CD8⁺ T cells in spleen and PLNs. b, Left, flow-cytometry analysis of the expression of CD62L and CD44, and right, quantification of the frequency of CD44^hiCD62L^lo cells, in conventional Foxp3^–CD4⁺ (top panels) or CD8⁺ T cells (bottom panels) from spleen and PLNs. c, Left panels, flow-cytometry analysis of the expression of IFNγ, IL-4 and IL-17, and right panels, quantification of frequencies of IFNγ⁺, IL-4⁺ and IL-17⁺ cells amongst conventional Foxp3^–CD4⁺ T cells from spleen and PLNs. d, Left, flow-cytometry analysis of the expression of IFNγ, and right, quantification of the frequency of IFNγ⁺ cells amongst CD8⁺ T cells from spleen and PLNs. e, Haematoxylin-and-eosin staining of spleen, PLNs, colon, pancreas, lung, liver and skin from age-matched Foxp3^CreScap^+/+ or Foxp3^CreScap^+/fl control and Foxp3^CreScap^fl/fl mice (28–35 weeks). f, g, Quantification of anti-nuclear antibodies (ANAs) (f) and anti-dsDNA antibodies (g) in the serum of Foxp3^CreScap^+/fl and Foxp3^CreScap^fl/fl mice (older than 3 months) (n = 23, Foxp3^CreScap^+/fl mice; n = 20, Foxp3^CreScap^fl/fl mice). h, Survival curve of Foxp3^CreScap^+/+ or Foxp3^CreScap^+/fl control (n = 17) and Foxp3^CreScap^fl/fl (n = 27) mice. **P < 0.01; two-tailed unpaired Student’s t-test (a–d, f, g). Data are means ± s.e.m. Data are compiled from two (a–e) independent experiments.

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