Extended Data Fig. 3: SCAP is dispensable for the function of Treg cells in autoimmune and acute inflammation.
From: Lipid signalling enforces functional specialization of Treg cells in tumours
a, Foxp3CreScap+/+ or Foxp3CreScap+/fl control (n = 13) and Foxp3CreScapfl/fl (n = 14) mice were immunized with MOG, and their EAE disease score was measured every day for 21 days. 0, no overt signs of disease; 1, limp tail; 2, limp tail plus hindlimb weakness; 3, total hindlimb paralysis; 4, hindlimb paralysis plus 75% of body paralysis (forelimb paralysis/weakness); 5, moribund. b, Mice as in a were euthanized in the recovery phase (days 22–25) of EAE for histological analysis of clinical disease. Left, haematoxylin-and-eosin staining of the spinal cord, and right, histological scores of the indicated regions of the brain and spinal cord (n = 9, Foxp3CreScap+/+ or Foxp3CreScap+/fl control mice; n = 10, Foxp3CreScapfl/fl mice). c, d, Foxp3CreScap+/+ or Foxp3CreScap+/fl control and Foxp3CreScapfl/fl mice were immunized with MOG to induce EAE (n = 5 mice in each group) and euthanized on day 16 for analysis. c, Cellularity of Foxp3– CD4+ (top) and CD8+ (bottom) T cells in PLNs, spleen and spinal cord. d, Percentages (left) and numbers (right) of IFNγ+ (top) and IL-17+ (bottom) Foxp3– CD4+ T cells from PLNs, spleen and spinal cord. e–h, Foxp3Cre/DTRScap+/+ or Foxp3Cre/DTRScap+/fl control and Foxp3Cre/DTRScapfl/fl mosaic mice were treated with diphtheria toxin (DT) (e, top, experimental design; n = 6 mice in each group) and euthanized 3 days after the final DT injection. e, Quantification of cellularity of Foxp3– CD4+ (lower left) and CD8+ (lower right) T cells. f, Quantification of the frequency of CD44hi CD62Llo cells in Foxp3– CD4+ (top) and CD8+ (bottom) T cells from spleen and PLNs. g, Quantification of frequencies of IFNγ+ (left), IL-4+ (middle) and IL-17+ (right) cells amongst Foxp3– CD4+ T cells from spleen and PLNs. h, Frequency of IFNγ+ cells amongst CD8+ T cells from spleen and PLNs. Two-tailed unpaired Student’s t-test (b–h) or two-way ANOVA (a). Data are means ± s.e.m. in a–h. Data are compiled from three (a) or two (b, e–h) independent experiments.
