Extended Data Fig. 4: SCAP/SREBP signalling maintains the functional state of Treg cells in the TME.
From: Lipid signalling enforces functional specialization of Treg cells in tumours
a–d, Foxp3CreScap+/+ or Foxp3CreScap+/fl control (n = 11) and Foxp3CreScapfl/fl (n = 14) mice were inoculated with B16 cells. a, b, Cellularity of CD8+ T cells (gated as CD8α+TCRβ+; a) and Foxp3–CD4+ T cells (b) in PLNs (left panels) and tumours (right panels; normalized to tumour weight to account for differences in tumour size between the genetic models) on day 19. c, d, Numbers of CD44hiCD62Llo (Tem) CD8+ cells (c) and CD44hiCD62Lhi (Tcm) CD8+ cells (d) from tumours. e, Foxp3CreScap+/+ or Foxp3CreScap+/fl control (n = 12) and Foxp3CreScapfl/fl (n = 14) mice were inoculated with B16 cells, and the number of Tim3+PD-1+ (Tex) CD8+ T cells from tumours was quantified. f, g, Foxp3CreScap+/+ or Foxp3CreScap+/fl control (n = 11) and Foxp3CreScapfl/fl (n = 14) mice were inoculated with B16 cells. Shown are flow-cytometry analysis of the expression of TNFα and IFNγ (left), and the relative frequencies of TNFα+ cells (middle) and IFNγ+ cells (right) amongst CD8+ T cells (f) and Foxp3–CD4+ T cells (g) from PLNs and tumours. h, Foxp3CreScap+/+ or Foxp3CreScap+/fl control and Foxp3CreScapfl/fl mice were inoculated with MC38 cells on day 0 and treated with anti-CD8 antibody on days –1, 2, 5, 8 and 11. Tumour growth was measured (n = 6 mice in each group). *P < 0.05; **P < 0.01; ***P < 0.001; two-tailed unpaired Student’s t-test (a–g) or two-way ANOVA (h). Data are means ± s.e.m. in a–h. Data are representative of two (h) or compiled from two (a–g) independent experiments.
