Fig. 4: Assessing antibody neutralization of ΔPRRA SARS-CoV-2.
From: Loss of furin cleavage site attenuates SARS-CoV-2 pathogenesis
a, Schematic for ΔPRRA SARS-CoV-2 reporter virus expressing mNeonGreen gene in place of ORF7. b, PRNT50 values measured by mNeonGreen expression, with wild-type SARS-CoV-2 on the x axis and ΔPRRA SARS-CoV-2 on the y axis. c–e, Representative curves from low (c), intermediate (d) and high (e) neutralizing sera from patients with COVID-19. n = 3. f–h, Neutralization curves from three monoclonal antibodies (mAb 1 (f), mAb 2 (g) and mAb 3 (h)). n = 3. i, Particle:PFU ratio determined from 40 fields, dividing into individual particles (left) and clusters (right) to determine ratios. j, Percentage of particles as individual virions (1), doubles (2) or larger clusters (>3). Data are mean ± s.e.m.
