Extended Data Fig. 2: ΔPRRA mutant processing and competition with wild-type SARS-CoV-2.
From: Loss of furin cleavage site attenuates SARS-CoV-2 pathogenesis
a, Quantification by densitometry of the full-length S (black) and S1/S2 cleavage form (grey) from western blot experiments in Vero E6 cells (n = 2). b, Schematic of RT–qPCR approach to detect deletion of the furin cleavage site. c, Primer curve validation with mixed wild-type/ΔPRRA plasmid ratio showing sensitivity. d, Deep sequencing results from ΔPRRA and wild-type competition assays based on percentage of total reads in that region (n = 3). e, Quantification by densitometry of the full-length S (black) and S1/S2 cleavage form (grey) from western blot experiments from Calu-3 cells (n = 2). f, Quantification by densitometry of the full-length S (black) and S1/S2 cleavage form (grey) from western blot experiments from Vero E6 cells expressing TMPRSS2 (n = 2). Data are mean.
