Extended Data Fig. 4: In vivo attenuation of ΔPRRA-mutant SARS-CoV-2 in K18-hACE2 mice.
From: Loss of furin cleavage site attenuates SARS-CoV-2 pathogenesis
a–d, Lung function evaluated using Flexivent for tissue damping (a), respiratory resistance (b), tissue elastance (c) and Newtonian resistance (d). In a–d, n = 10 mice infected with wild-type SARS-CoV-2, and 8 mice infected with ΔPRRA SARS-CoV-2. e, Whole lung histopathology sections at 7 dpi, from mock-infected mice (i), or mice infected with wild-type (j) or ΔPRRA (j) SARS-CoV-2, with the least (left) and most (right) severe sections as representative samples from 3 mock-infected, 2 wild-type-infected and 3 ΔPRRA-infected mice. f, Chemokine and cytokine analysis of mouse lung at 7 days after mock infection (white), or at 7 dpi with wild-type (black) or ΔPRRA SARS-CoV-2. n = 8 mice for all groups. P values from two-tailed Mann–Whitney (a–d) or two-tailed Student’s t-tests (f), relative to control. Data are mean ± s.e.m. Scale bar, 5 mm.
