Extended Data Fig. 6: Construction of CCL22-KI-tdTomato mice.
From: Affinity-coupled CCL22 promotes positive selection in germinal centres
a, The CRISPR–Cas9 strategy for IRES-tdTomato insertion into Ccl22. The targeting sgRNA (red line), genotyping primers (yellow arrowheads) and the insert segment (red block) are shown in the context of genomic sequences (nucleotide coordinates according to NCBI). b, Representative genotyping PCR results for the wild-type and KI mutant allele. c, Gating of CD11c+ dendritic cells in lymph node cells (left) and contour plots to show tdTomato reporter fluorescence from CCL22-KI-tdTomato or control B6 mice (right).
