Extended Data Fig. 7: Characteristics of GC B cells and serum antibody affinities in the absence of B cell CCL17 and CCL22.
From: Affinity-coupled CCL22 promotes positive selection in germinal centres
a, Representative contour plots of NIP-binding GC B cells of μMT (80%):wild-type (20%) or μMT (80%):DKO (20%) bone marrow chimaera. b, Summary statistics of fractions of NIP-binding GC B cells at indicated time points. Each dot indicates one mouse, and lines denote means. Data are pooled from three independent experiments. P values by two-tailed t-tests. c, Representative contour plots of IgM+ and IgG1+ GC B cells of μMT (80%):wild-type (20%) or μMT (80%):DKO (20%) bone marrow chimaera. d, e, Summary statistics of IgM+ (d) and IgG1+ (e) GC B cells at day 13 post-immunization. Each dot indicates one chimaeric mouse, and lines denote means. Data are pooled from three independent experiments. P values by two-tailed t-tests. f, Representative ELISA titration curves of serum IgG binding to NP4- or NP30-BSA 21 days post-immunization. g, Ratios between NP4-binding and NP30-binding EC50. Each symbol indicates one mouse, and lines denote means. Data are pooled from three independent experiments. The P value by two-tailed t-test.
